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Frequently Asked Questions (FAQ)

Question. which type of NextGen CelBlokingTM kit should be used when?

Answer. Nano- NextGen CelBlokingTM units can be used for any type of cytology specimens, including hypocellular specimens. Even other specimens such as curettages and brush biopsies could be cell-blocked with Nano units.

https://youtu.be/y29SS1NwO_8

https://youtu.be/ZPb0nq8MsLk

However, Micro- NextGen CelBlokingTM units should only be used for more than 1 ml of concentrated cytology specimens with high cellularity with Tissuecrit* of more than 50%. The results with hypocellular specimens will not be optimum with the Micro version.

Tissuecrit* Guesstimated proportion of sediment in relation to the supernatant in the concentrated specimen.

https://youtu.be/i-ZpXaljiIs

https://youtu.be/TRW5Vswy6J8

 

Question. what is Tissuecrit?

Answer. Tissuecrit is based on the concept of Hematocrit of blood. It is guesstimated proportion of sediment in relation to the supernatant in the concentrated specimen.

 

Question. what is AV Marker?

Answer. AV Marker is a dark colored marker used for identifying the level at which the diagnostic material in the cell-blocks is located in the wells. It is a guide to monitor the depth of cutting of the cell-block paraffin sections by the histotechnologist. It is visible on the paraffin sections and so allows the histotechnologist to orient the sections in an identical manner on different slides in serial order as part of the SCIP approach. AV markers are precisely set the level of the bottom of the wells in the medium discs of Nano- and Micro- version of NextGen CelBlokingTM units.

 

Question. Do we have to remove the Tissue paper cover put on the opening of the wells with sediments during embedding in paraffin?

Answer. No, the Tissue paper cover put on the opening of the wells with sediments during embedding in paraffin need NOT be removed. The medium disc of the cell-block after tissue processing is embedded in such a way that the bottom of the wells is below to become the cutting surface and the side with Tissue paper cover would be inside the paraffin block opposite to the cutting surface nearer to the bottom of the wells. Please see the video explaining this at https://youtu.be/h2yuWg8H4pM

 

Question. Why the results with blood rich specimens are not good?

Answer. For the specimens with a high proportion of blood contamination, please lyse the blood first (using BloodLyzTM reagent kit www.AVBioInnovation.com) and then make cell block from the concentrated specimen with a predominance of the diagnostic component.

 

Question. What do I do if I do not have centrifuge with free swinging rotor?

Answer. If centrifuge with a free-swinging rotor is not available, the sedimentation steps used in the procedure twice could be replaced by allowing the sedimentation to be achieved by gravity. For these steps otherwise needing centrifugation, let the Nano units be left undisturbed for 30 minutes in cold (such as in a refrigerator, DO NOT allow to freeze). Then discard the supernatant gently with the help of transfer pipette (just inverting the Nano unit to discard the supernatant may not be safe because the sediments by gravity may not be compact as achieved by centrifugation).

 

Question. What do I do if I do not have centrifuge rotor for 50 ml conical tube and Nano unit cannot be accommodated in the rotor cups?

Answer. If centrifuge for 50 ml tubes is not available, the sedimentation steps used in the procedure twice could be replaced by allowing the sedimentation to be achieved by gravity. For these steps otherwise needing centrifugation, let the Nano units be left undisturbed for 30 minutes in cold (such as in a refrigerator, DO NOT allow to freeze). Then discard the supernatant gently with the help of transfer pipette (just inverting the Nano unit to discard the supernatant may not be safe because the sediments by gravity may not be compact as achieved by centrifugation).

 

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